Journal: Journal of Ginseng Research

Article Title: Stereospecific anticancer effects of ginsenoside Rg3 epimers isolated from heat-processed American ginseng on human gastric cancer cell

doi: 10.1016/j.jgr.2013.11.007

Figure Lengend Snippet: Changes in effects of American ginseng upon heat-processing on AGS cell proliferation. (A) Morphological changes were confirmed using phase-contrast microscopy. (B) Cells were treated with American ginseng with or without heat-processing at different concentrations (150 μg/mL, 300 μg/mL, and 600 μg/mL) for 24 h. Relative cell proliferation was measured by the CCK-8 assay. Each value represents the mean ± standard deviation of three independent experiments. (C) Schematic description for the preparation of methanol-soluble fraction with HP 20 column chromatography. (D) Cells were treated with water eluate or methanol eluate at different concentrations (12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL) for 24 h. Relative cell proliferation was measured by the CCK-8 assay. Each value represents the mean ± standard deviation of three independent experiments. * p < 0.05 compared with the vehicle control. CCK-8, Cell Counting Kit-8.

Article Snippet: The human gastric cancer AGS cell line was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Microscopy, CCK-8 Assay, Standard Deviation, Column Chromatography, Control, Cell Counting

Journal: Journal of Ginseng Research

Article Title: Stereospecific anticancer effects of ginsenoside Rg3 epimers isolated from heat-processed American ginseng on human gastric cancer cell

doi: 10.1016/j.jgr.2013.11.007

Figure Lengend Snippet: Changes in effects of ginsenosides Rb1 and Re upon heat-processing on AGS cell proliferation. (A) Cells were treated with ginsenoside Rb1 or heat-processed ginsenoside Rb1 at different concentrations (12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL) for 24 h. (B) Cells were treated with ginsenoside Re or heat-processed ginsenoside Re at different concentrations (12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL) for 24 h. Relative cell proliferation was measured by the CCK-8 assay. Each value represents the mean ± standard deviation of three independent experiments. (C) HPLC chromatogram of ginsenoside Rb1 prior to heat-processing. (D) HPLC chromatogram of ginsenoside Rb1 after heat-processing. * p < 0.05 compared with vehicle control. CCK-8, Cell Counting Kit-8; HPLC, high performance liquid chromatography.

Article Snippet: The human gastric cancer AGS cell line was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: CCK-8 Assay, Standard Deviation, Control, Cell Counting, High Performance Liquid Chromatography

Journal: Journal of Ginseng Research

Article Title: Stereospecific anticancer effects of ginsenoside Rg3 epimers isolated from heat-processed American ginseng on human gastric cancer cell

doi: 10.1016/j.jgr.2013.11.007

Figure Lengend Snippet: Anticancer effects of ginsenosides on AGS cell proliferation. (A) Cells were treated with ginsenoside 20( S,R )-Rg3 at different concentrations (12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL) for 24 h. (B) Cells were treated with ginsenoside Rg5/Rk1 at different concentrations (12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL) for 24 h. (C) Cells were treated with ginsenoside 20( S )-Rg3 at different concentrations (6.25 μg/mL, 12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL) for 24 h. (D) Cells were treated with ginsenoside 20( R )-Rg3 at different concentrations (6.25 μg/mL, 12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL) for 24 h. Relative cell proliferation was measured by the CCK-8 assay. Each value represents the mean ± standard deviation of three independent experiments. * p < 0.05 compared with the vehicle control. CCK-8, Cell Counting Kit-8.

Article Snippet: The human gastric cancer AGS cell line was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: CCK-8 Assay, Standard Deviation, Control, Cell Counting

Journal: BMC Cancer

Article Title: Upregulation of bone morphogenetic protein 1 is associated with poor prognosis of late-stage gastric Cancer patients

doi: 10.1186/s12885-018-4383-9

Figure Lengend Snippet: BMP1 inhibitor UK 383367 inhibits mobility of gastric cancer cell line AGS and MKN45. ( a ) Relative BMP1 expression of AGS, MKN28, and MKN45 cells was determined by qRT-PCR and immunoblotting. ( b ) MKN28 cells were seeded into a culture insert for 24 h, followed by culturing with 2% serum plus UK 383367 for another 24 h. After removal of the culture insert, images were taken 0 and 8 h using an Olympus FV1000 laser confocal scanning microscope equipped with differential interference contrast system. ImageJ was used to measure the distance covered by migrating cells, and statistical analysis was performed using Student’s t-test. ( c ) AGS cells was treated with 400 nM, 800 nM, or 1600 nM UK 3367, and the mobility of treated cells was assessed by wound healing assay as described in ( b ). ( d ) The mobility of MKN45 was assayed as described above. ( e ) MKN45 cells were seeded into the upper chamber of a transwell with or without UK 383367 and cultured for 24 h. Serum was then removed from the upper chamber, and the cells were allowed to move for 24 h. Cells were then fixed, stained with DAPI, and imaged using an Olympus FV1000 laser confocal scanning microscope. The percentage of the cells migrating to the lower chamber was calculated. Two independent experiments were carried out

Article Snippet: Gastric cancer cell line AGS [ ] (BCRC number 60102) was obtained from Bioresource Collection and Research Center, Hsinchu, Taiwan.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Microscopy, Wound Healing Assay, Cell Culture, Staining

Journal: Molecules

Article Title: Phytochemical Profiling and Biological Activities of Extracts from Bioreactor-Grown Suspension Cell Cultures of Schisandra henryi

doi: 10.3390/molecules29225260

Figure Lengend Snippet: The viability of the cancer AGS, HeLa, and SKOV-3 cells and the non-cancer fibroblasts after 24 h of treatment with the S. henryi extracts from suspension cultures grown in a bioreactor. The control (ctrl) was the cells with DMSO (0.75% ( v / v )). Two independent experiments were conducted, with six repetitions (n = 12) of the MTT assay. The results are shown as the mean values with standard deviations (±SDs, error bars). Significant differences relative to the control are marked with an asterisk “*” ( p < 0.05).

Article Snippet: Human gastric cancer AGS, ovarian cancer SKOV-3, and cervical adenocarcinoma HeLa S3 cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Suspension, Control, MTT Assay